Before facility inductions and practical training can commence, all clients need to undertake and follow licensing steps. These include watching the Online Tutorials, Read the AMREP Flow users syllabus, Take the AMREP Quiz. Once all these are successfully completed, our clients can request further training.
AMREP Flow Cytometry Core Facility provides free of charge comprehensive training on:
Basic theory of flow cytometry; including fluidics, optics and how data is generated.
Instrument operation; including correct start-up, shut-down and trouble shooting.
Sample preparation - good data generation is impossible without careful sample preparation. AMREP Flow staff are more than happy to discuss topics such as cell preparation and staining in order for you generate good data, and importantly, prevent wasting your time and money through poor sample preparation.
All users must be fully licensed with the flow facility before they are permitted to use any of the analysing instruments.
In addition, it has been supported that users who have a sound understanding of the concepts covered in training are better equipped to troubleshoot their own problems and generally produce higher quality data.
Training will consist of 3-4 separate sessions where you will be taught how to correctly use the instruments and the basics of setting up a 4-colour flow experiment.
Licensing step 1. watch the online tutorial videos before we commence your flow training. During training there will be a few follow up questions in regards to these videos.
Licensing step 2. Download and read the Syllabus
Licensing step 3. Quiz
Licensing step 4. Speak with your supervisor and decide which instrument will be most suited for your scientific applications (Specs of our instruments can be found here) and contact us at [email protected] to book in a time for that particular instrument to start your practical training sessions. Training sessions will continue until you can independently perform a 4-colour flow experiment including compensation and will finish with one of our staff members overseeing your own first flow experiment.
Our website is full of useful research links that are well worth checking out, including CD charts, spectral viewers etc... under the Education tab.
Training is free, and a refresher session is always welcomed if you have not performed flow experiments in some time.
Geza, Eva, Maggie and Steve are also happy to provide guidance on polychromatic panel design and other flow topics after your training is completed, however please be aware that we must be acknowledged in any published work for help given.