Flow Sorters AMREPFlow core flow cytometry facility


The AMREP Flow Facility houses two BD FACS Aria instruments (with up to 4-way sorting) and a BD Influx (with up to 6-way sorting). The Arias have a 3 laser (488nm, 633nm & 405nm) 9 colour detection capability. The Influx has 4 lasers (488nm, 633nm, 405nm & 561nm) 14 simultaneous colour detection capability. PC3 Aria users please note changes to OGTR regulations as of 2014.

The FACS sorting instruments are available from 10am to 5:30pm Monday to Friday (Monash users please note this excludes Victorian Public Holidays). Operation outside these times may be possible by prior arrangement - please Contact Us for information.

Please find sample preperation guidline for sorting here

BD Influx

The Influx is capable of sorting up to 6 cell populations simultaneously and can operate from a 70, 86 or 100 micron nozzle and, depending on cell type and sample preparation, is able to sort up to 36 million cells per hour. Please contact our facility in order to discuss the optimum sorting conditions for your experiment. Control and sort sample preparations must be in polypropylene tubes. The Influx is capable of sorting into 50ml (for 2 way), 15ml (for 2 way), 5ml (for 2 or 4 way) and cell culture plates (6-well, 12-well, 24-well, 48-well and 96-well).

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The table below is a guide only and indicates some of the most used fluorochromes on the Influx. Note: The last channel off the violet laser can have three different filter options which include 660/20 710/50 and the default 750LP. It is recommended that you approach a member of the Flow Facility when designing an experiment in order to discuss fluorochrome harmony.

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BD FACSAria

The Aria sorters operate from a 70 micron nozzle only. Depending on cell type and sample preparation, are able to sort up to 36 million cells per hour. Please contact our facility in order to discuss the optimum sorting conditions for your experiment. Both sorters are capable of sorting into 15ml (for 2 way) 5ml (for 2 or 4 way) and cell culture plates (6-well, 12-well, 24-well, 48-well and 96-well). NOTE: Due to OGTR changes, we are now able to offer PC2 sorts within the PC3 environment. You can take your uninfected human or murine cells out of PC3 into PC2!!

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The table below indicates some of the most used fluorochromes on the ARIA. The table should be used as a guide only and it is recommended that you approach a member of the Flow Facility when designing an experiment in order to discuss fluorochrome harmony.

Please be aware:

This is not a comprehensive list. If you wish to use a fluorochrome that is not listed below please contact us to discuss its compatibility with the instrument. *may need a filter change to detect, please make a request before booking your sort. Image Alt Text