Flow Analysers AMREPFlow core flow cytometry facility


AMREP Flow houses a total of 8 Flow analysis instruments over 3 buildings, all with varying capabilities and applications. Comprehensive training for all instruments is provided free of charge to enable unsupervised researcher operation. Training culminates with a licensing 'test', all prospective clients need to undergo induction, training and licensing before bookings or unsupervised use of the instruments are allowed. All users must be aware of and follow the Standard Operating Procedures (SOPs) for each instrument. SOPs are accessible under each instrument specification on this page. How do you become licensed? Please contact the whole team for details.

Please find sample preperation guidline for analysis here

If you are planning on using and performing regular immunophenotyping on primary human samples on any of the AMREPFlow instruments, the sample MUST be fixed. (Fixation protocol under instrument SOPs and Suggested Protocols - Education tab) If you require to run and analyze primary human samples that are NOT FIXED for functional/kinetic etc. studies, you MUST submit a Risk Assessment Document and an SWI to AMREPFlow, as the study will have to be approved by the AMREPFlow OHS Committee. Thank you for your cooperation and for making our/your working environments safer.

BD LSR-Fortessa X-20

Image Alt Text

Image Alt Text

Image Alt Text CLICK HERE for Fortessa X-20 Compensation Matrix Image Alt Text CLICK HERE for Fortessa X-20 Spillover Spreading Matrix

Image Alt Text CLICK HERE for Fortessa X-20 SOP Image Alt Text CLICK HERE for Fortessa X-20 HTS SOP

BD LSR II and LSRForessa

Image Alt Text Image Alt Text

Image Alt Text

Image Alt Text CLICK HERE for Fortessa Compensation Matrix Image Alt Text CLICK HERE for Fortessa Spillover Spreading Matrix

Image Alt Text CLICK HERE for LSR Compensation Matrix Image Alt Text CLICK HERE for LSR Spillover Spreading Matrix

Image Alt Text CLICK HERE for LSR-II SOP Image Alt Text CLICK HERE for Fortessa SOP Image Alt Text CLICK HERE for Fortessa HTS SOP

BC CytoFlex

Image Alt Text

Image Alt Text

Image Alt Text CLICK HERE for CtyoFLEX SOP

BD FACS Canto II

Image Alt Text

Image Alt TextImage Alt Text

Image Alt Text CLICK HERE for Monash Canto Compensation Matrix Image Alt Text CLICK HERE for Monash Canto Spillover Spreading Matrix

Image Alt Text CLICK HERE for Burnet Canto Compensation Matrix Image Alt Text CLICK HERE for Burnet Canto Spillover Spreading Matrix

Image Alt Text CLICK HERE for Canto-II SOP Image Alt Text CLICK HERE for CantoII HTS SOP

BD FACS Calibur - Three Available on Campus

Image Alt Text

Image Alt Text Image Alt Text

Image Alt Text CLICK HERE for Calibur SOP