Suggested Protocols AMREPFlow core flow cytometry facility
If you are planning on using and performing regular immunophenotyping on primary human samples on any of the AMREPFlow instruments, the sample MUST be fixed. (Fixation protocol under instrument SOPs and Suggested Protocols - Education tab)
If you require to run and analyze primary human samples that are NOT FIXED for functional/kinetic etc. studies, you MUST submit a Risk Assessment Document and an SWI to AMREPFlow, as the study will have to be approved by the AMREPFlow OHS Committee.
Thank you for your cooperation and for making our/your working environments safer.
eBioscience Flow Cytometry Protocols - very useful
Bio-Rad Flow Cytometry Protocols - very useful
Bio-Rad Flow Cytometry Intracellular Staining Flow Tips - very useful
Bio-Rad - Human, Mouse Antigen Density Calculations
Moores Cancer Centre, UCSan Diego Flow Cytometry Protocols - very useful
Abcam - Propidium Iodide staining
Recommended antibiotics for tissue culture
A guide to Flow Cytometry Cell Cycle Analysis
Live Dead and Fixable Dyes - STEMCell Technologies
Live Dead and Fixable Dyes - Thermo Fisher
Live Dead and Fixable Dyes - BioLegend
ACCUMAX-Innovative Cell Technologies
Protocol for the use of ACCUMAX™ in Primary Tissue Dissociation
