Suggested Protocols AMREPFlow core flow cytometry facility


If you are planning on using and performing regular immunophenotyping on primary human samples on any of the AMREPFlow instruments, the sample MUST be fixed. (Fixation protocol under instrument SOPs and Suggested Protocols - Education tab)

If you require to run and analyze primary human samples that are NOT FIXED for functional/kinetic etc. studies, you MUST submit a Risk Assessment Document and an SWI to AMREPFlow, as the study will have to be approved by the AMREPFlow OHS Committee.

Thank you for your cooperation and for making our/your working environments safer.

eBioscience Flow Cytometry Protocols - very useful

Bio-Rad Flow Cytometry Protocols - very useful

Bio-Rad Flow Cytometry Intracellular Staining Flow Tips - very useful

Bio-Rad - Human, Mouse Antigen Density Calculations

Moores Cancer Centre, UCSan Diego Flow Cytometry Protocols - very useful

Abcam - Propidium Iodide staining

Recommended antibiotics for tissue culture

A guide to Flow Cytometry Cell Cycle Analysis

Live Dead and Fixable Dyes - STEMCell Technologies

Live Dead and Fixable Dyes - Thermo Fisher

Live Dead and Fixable Dyes - BioLegend

StemPro® Accutase®

ACCUMAX-Innovative Cell Technologies

Protocol for the use of ACCUMAX™ in Primary Tissue Dissociation

ACCUMAX™-Millipore

MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments