The Facility
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Welcome to AMREP Flow-Cytometry Core Facility,
The AMREP (Alfred Medical Research and Education Precinct) Flow Cytometry Core Facility is a state of the art, world class cell sorting and cell analysis laboratory, catering for the scientific research community based at AMREP and broader Melbourne. The facility is located at AMREP, Commercial Rd., Melbourne, specifically in the department of Immunology/Pathology Monash building 2nd floor, and has some instruments located at the Burnet Institute. Managed by Mr. Geza Paukovics, B.Med. Lab. Sci., with 18 years of extensive experience in the field of flow cytometry and cell sorting, the facility offers comprehensive training and education, experimental design and protocol guidance specifically targeting effective data generation and interpretation in the field of flow cytometry. The facility offers services catering for both animal and human cell sorting in a PC2 environment. Uniquely, the facility also caters for infectious sample sorting (eg. HIV, HepC) in a dedicated PC3 environment. The facility is equipped with two high throughput cell sorters, and five cell analysis platforms. These instruments may only be booked by qualified and licensed users. ALL users must be familiar with the booking rules and costs, usage guidelines and standard operating procedures (SOPs). For more information please do not hesitate to contact us.
Location
The AMREP Flow Cytometry Facility's Core Laboratory is located on the 2nd Floor of the Monash Immunology/Pathology Department, headed by Prof. Fabienne Mackay. However the easiest way to locate us would be at 85 Commercial Rd Melbourne 3004, Burnet Institute reception and ask to contact Geza Paukovics or Michael Thomson. Please see contact details.
Staff
Geza Paukovics - AMREP Senior Flow Cytometrist
Mr. Geza Paukovics started his tenure at the Burnet Insitute in 1992, which then was located on the grounds of Fairfield Hospital and associated with the Victorian Infectious Diseases Reference Laboratory (VIDRL). Having worked extensively as a research assistant for a number of years, he was given the opportunity to run the Burnet Institute Flow-Cytometry Facility by Prof. Suzanne Crowe in 1997. Geza would like to acknowledge expert guidance and tuition in the field of flow cytometry by Steven Hunter and during his one year secondment to Walter Eliza Hall Institute (WEHI) by Dr. Frank Battye. Having graduated from the "old School" of flow cytometry, where manual LASER and instrument alignment was commonly practiced, he has extensive knowledge of flow cytometry principles, theory, data generation and data interpretation. Through intellectual input into experimental projects, Geza has appeared on numerous publications. While running the Burnet facility, Geza was invited to perform after hours cell sorting at the Monash Central Medical School by Dr. Richard Boyd. Maintaining contact with the Monash facility and upon Burnet Institute's migration to AMREP, Geza was given the role of managing the newly established AMREP Flow Cytometry Core Facility. Geza takes pride in the Flow Cytometry Department, particularly the department's role in cell sorting and cell analysis, hence paramount importance is placed on staff induction into the facility, staff education and tuition which culminates in users being licensed upon exhibiting competence of instrument usage and data generation. The AMREP Flow Department's education extends to organising technical seminars, invited experts in the field as well as associated technolgies. Geza would like to acknowledge ongoing support for the facility by Prof. Suzanne Crowe and Dr. Paul Cameron and also Mr. Michael Thomson for augmenting the capabilities of the facility and providing an excellent working atmosphere.
Michael Thomson - AMREP Flow Cytometrist
Michael graduated with a Bachelor of Applied Science (Biochemistry/Chemistry) in 2002 from Swinburne University. Whilst completing the degree, Michael gained lab experience at the Walter & Eliza Hall Institute, working in the Media Preparation Division, Monoclonal Antibody Facility and Immunology Division, before taking up full time work in the Immunology Division upon graduation, where he first experienced flow cytometry by isolating and staining splenocytes for sorting and analysis. After taking an extended break to travel, Michael returned to laboratory work in 2006 taking up a position in the Tissue Repair Group at the Australian Stem Cell Centre (ASCC), isolating and preparing mouse osetocytes for sorting and analysis by flow cytometry. Michael began in the AMREP Flow Cytometry Facility in 2009 aiding Geza in operating the laboratory, as well as completing a Masters in Biotechnology & Business there which he began at the ASCC.
