AMREP Flow Core Facility NEWS and UPDATES
Retiring & Upgrading Calibur computers:
We are in the process of upgrading all the Calibur computers to G4 MACS.
Over the next three week (until Friday 21st June) please back-up and remove your data from all Caliburs (Mad Mongoose, Uncle Joe & Burnet ARI) as we will be retiring the current computers.
Cheers,
AMREPFlow Staff
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We'll try and give you interesting and important information as we come across it, either from the Flow Cytometry mailing list or relevant vendors:
This one comes from eBio:
http://www.ebioscience.com/resources/faq/flow-cytometry-faq.htm
Q. I read a paper regarding incompatibility of PE-Cy5.5 conjugates and DEC205+ cells. What alternative options are available?
CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates (Part et al. 2012 J. Immunol Methods 384:184-90); therefore, this format should not be used on CD205+ dendritic cells. We recommend alternative formats such as PerCP-Cy5.5 or PerCP-eFluor 710 whenever possible.
Thanks to all that attended the AMREP Flow Users meeting. Slides and feedback can be found here.
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A number of updated and very useful links are found under the Flow Cytometry Information & Tips Education & Useful Links tab including FLUORESCENCE SPECTRAL VIEWERS and SPECTAL GUIDES to assist in multicolour panel design and some neat interactive CELL MARKER information to assist in identifying your cells.
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Uncle Joe has still got intermittent com problems - not 100% but ok to use from cold start.
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Old topics have been moved to the forum. Please browse & use it: it's designed as an open means of communication.
